| 19:30 | | Newcastle Benfield | | Seaham Red Star | | 1:1, pen. 4:3 | | |
| Newcastle upon Tyne | | |
| 19:30 | | Easington Sports | | Tring Athletic | | 2:0 | | |
| Easington | | |
| 19:30 | | Ardley United | | Edgware Town | | 0:0 | | |
| Ardley | | |
| 19:30 | | Leighton Town | | Abingdon United | | 3:0 | | |
| Barton-le-Clay | | |
| 19:30 | | East Preston | | Chatham Town | | 0:2 | | |
| East Preston | | |
| 19:30 | | Crowborough Athletic | | Crawley Down Gatwick | | 3:4 | | |
| Crowborough | | |
| 19:30 | | AFC Uckfield Town | | Little Common | | 0:1 | | |
| Uckfield | | |
| 19:30 | | Lancing | | Phoenix Sports | | 3:3, pen. 10:11 | | |
| Lancing | | |
| 19:30 | | Pagham | | Fleet Town | | 2:1 | | |
| Pagham | | |
| 19:30 | | Cowes Sports | | Totton & Eling | | 1:0 | | |
| Cowes | | |
| 19:30 | | Buckland Athletic | | Bitton | | 1:1, pen. 3:5 | | |
| Newton Abbot | | |
| 19:30 | | Wells City | | Bovey Tracey | | 2:0 | | |
| Wells | | |
| 19:30 | | Shepton Mallet | | Torrington | | 3:0 | | |
| Shepton Mallet | | |
| 19:30 | | Brislington | | Cadbury Heath | | 0:2 | | |
| Brislington | | |
| 19:45 | | Penrith | | Pickering Town | | 3:2 | | |
| Penrith | | |
| 19:45 | | Northallerton Town | | Billingham Town | | 1:4 | | |
| Northallerton | | |
| 19:45 | | Sunderland RCA | | Durham City | | 3:1 | | |
| Sunderland | | |
| 19:45 | | Whickham | | West Allotment Celtic | | 0:0, pen. 2:4 | | |
| Whickham | | |
| 19:45 | | Glasshoughton Welfare | | Knaresborough Town | | 1:1, pen. 2:4 | | |
| Glasshoughton | | |
| 19:45 | | Hebburn Town | | Hemsworth Miners Welfare | | 4:1 | | |
| Hebburn | | |
| 19:45 | | Consett AFC | | Ryhope Colliery Welfare | | 3:2 | | |
| Consett | | |
| 19:45 | | Marske United | | North Shields | | 3:0 | | |
| Marske-by-the-Sea | | |
| 19:45 | | Garforth Town | | Whitley Bay | | 0:3 | | |
| Garforth | | |
| 19:45 | | Ashington | | Goole AFC | | 2:1 | | |
| Ashington | | |
| 19:45 | | 1874 Northwich | | Warrington Rylands 1906 | | 1:6 | | |
| Winsford | | |
| 19:45 | | Longridge Town | | Winsford United | | 2:1 | | |
| Longridge | | |
| 19:45 | | Wythenshawe Amateurs | | Shelley Community | | 1:1, pen. 4:2 | | |
| Manchester | | |
| 19:45 | | Runcorn Town | | Thackley | | 2:1 | | |
| Runcorn | | |
| 19:45 | | Eccleshill United | | Silsden AFC | | 0:3 | | |
| Wrose | | |
| 19:45 | | AFC Darwen | | Barnoldswick Town | | 2:4 | | |
| Darwen | | |
| 19:45 | | Northwich Victoria | | Padiham | | 1:0 | | |
| Northwich | | |
| 19:45 | | Daisy Hill | | Colne | | 0:2 | | |
| Westhoughton | | |
| 19:45 | | Athersley Recreation | | Charnock Richard | | 1:4 | | |
| Athersley | | |
| 19:45 | | Irlam | | Liversedge | | 2:1 | | |
| Salford | | |
| 19:45 | | Maine Road | | Squires Gate | | 0:5 | | |
| Manchester | | |
| 19:45 | | Congleton Town | | Burscough | | 1:1, pen. 3:2 | | |
| Congleton | | |
| 19:45 | | Hanley Town | | Lye Town | | 3:1 | | |
| Hanley | | |
| 19:45 | | Rugby Town | | Worcester City | | 0:2 | | |
| Rugby | | |
| 19:45 | | Sporting Khalsa | | Boldmere St Michaels | | 2:1 | | |
| Willenhall | | |
| 19:45 | | Highgate United | | AFC Bridgnorth | | 0:3 | | |
| Solihull | | |
| 19:45 | | Stourport Swifts | | Coventry United | | 1:3 | | |
| Stourport-on-Severn | | |
| 19:45 | | Gresley Rovers | | Wellington | | 2:0 | | |
| Church Gresley | | |
| 19:45 | | Stone Old Alleynians | | Tividale | | 0:0, pen. 1:4 | | |
| Stone | | |
| 19:45 | | Bewdley Town | | Black Country Rangers | | 1:5 | | |
| Bewdley | | |
| 19:45 | | Romulus | | Coventry Sphinx | | 0:0, pen. 3:4 | | |
| Sutton Coldfield | | |
| 19:45 | | Chelmsley Town | | Shifnal Town | | 0:3 | | |
| Coleshill | | |
| 19:45 | | Westfields | | Brocton | | 2:0 | | |
| Hereford | | |
| 19:45 | | Whitchurch Alport | | Haughmond | | 3:1 | | |
| Whitchurch | | |
| 19:45 | | Racing Club Warwick | | AFC Wulfrunians | | 3:0 | | |
| Warwick | | |
| 19:45 | | Anstey Nomads | | Sleaford Town | | 3:0 | | |
| Anstey | | |
| 19:45 | | Lutterworth Town | | Staveley Miners Welfare | | 2:0 | | |
| Lutterworth | | |
| 19:45 | | Radford | | Shepshed Dynamo | | 1:1, pen. 3:4 | | |
| Nottingham | | |
| 19:45 | | Spalding United | | Barton Town FC | | 1:3 | | |
| Spalding | | |
| 19:45 | | Quorn | | Melton Town | | 2:0 | | |
| Quorn | | |
| 19:45 | | AFC Mansfield | | Sherwood Colliery | | 2:1 | | |
| Clipstone The Forest Town Stadium | | |
| 19:45 | | Long Eaton United | | Grimsby Borough | | 3:1 | | |
| Long Eaton | | |
| 19:45 | | Newark | | Deeping Rangers | | 4:0 (1:0) | | |
| Newark Lowfields | | |
| 19:45 | | Maltby Main | | Handsworth | | 2:1 | | |
| Maltby | | |
| 19:45 | | West Bridgford | | Dunkirk | | 1:1, pen. 5:3 | | |
| West Bridgford Regatta Way Sports Ground | | |
| 19:45 | | Leicester Nirvana | | Oadby Town | | 1:3 | | |
| Leicester | | |
| 19:45 | | Long Melford | | Northampton ON Chenecks | | 3:2 | | |
| Long Melford | | |
| 19:45 | | Whitton United | | Ipswich Wanderers | | 4:3 | | |
| Ipswich | | |
| 19:45 | | Newmarket Town | | Walsham-le-Willows | | +:- | | |
| Newmarket | | |
| 19:45 | | Hadleigh United | | Mildenhall Town | | 3:4 | | |
| Hadleigh | | |
| 19:45 | | Peterborough Northern Star | | Potton United | | 0:1 | | |
| Peterborough | | |
| 19:45 | | Diss Town | | Framlingham Town | | 1:0 | | |
| Diss | | |
| 19:45 | | Thetford Town | | Wellingborough Town | | 2:0 | | |
| Blofield | | |
| 19:45 | | Burton Park Wanderers | | AFC Sudbury | | -:+ | | |
| Burton Latimer | | |
| 19:45 | | Gorleston | | Swaffham Town | | 5:0 | | |
| Gorleston | | |
| 19:45 | | Haverhill Rovers | | Norwich United | | 2:1 | | |
| Haverhill | | |
| 19:45 | | Eynesbury Rovers | | Desborough Town | | 7:3 | | |
| St Neots | | |
| 19:45 | | Wellingborough Whitworth | | Harborough Town | | 1:6 | | |
| Wellingborough | | |
| 19:45 | | Woodbridge Town | | Biggleswade United | | 2:2, pen. 6:7 | | |
| Woodbridge | | |
| 19:45 | | Stowmarket Town | | Rothwell Corinthians | | 2:1 | | |
| Stowmarket | | |
| 19:45 | | Wroxham | | Arlesey Town | | 5:0 | | |
| Wroxham | | |
| 19:45 | | Redbridge | | Harpenden Town | | 0:2 | | |
| London | | |
| 19:45 | | Harlow Town | | Enfield 1893 | | 5:1 | | |
| Harlow | | |
| 19:45 | | Clapton | | Sporting Bengal United | | 2:2, pen. 5:6 | | |
| Forest Gate | | |
| 19:45 | | Hoddesdon Town | | Clacton | | 1:2 | | |
| Hoddesdon | | |
| 19:45 | | New Salamis | | Colney Heath | | 1:0 | | |
| London | | |
| 19:45 | | Southend Manor | | Ware | | 0:5 | | |
| Southend-on-Sea | | |
| 19:45 | | Walthamstow | | Maccabi London Lions | | 3:2 | | |
| London | | |
| 19:45 | | Saffron Walden Town | | Little Oakley | | 4:2 | | |
| Saffron Walden | | |
| 19:45 | | Stansted | | Takeley | | 3:2 | | |
| Stansted Mountfitchet | | |
| 19:45 | | Cockfosters | | Stanway Rovers | | 4:2 | | |
| London | | |
| 19:45 | | Hullbridge Sports | | Hadley | | 0:2 | | |
| Hullbridge | | |
| 19:45 | | Sawbridgeworth Town | | Romford | | 2:1 | | |
| Sawbridgeworth | | |
| 19:45 | | Brantham Athletic | | Benfleet | | 1:1, pen. 3:2 | | |
| Brantham | | |
| 19:45 | | Clanfield | | Long Crendon | | 0:1 | | |
| Clanfield | | |
| 19:45 | | Leverstock Green | | Wembley | | 3:1 | | |
| Leverstock Green | | |
| 19:45 | | Tuffley Rovers | | Harefield United | | 2:5 | | |
| Tuffley | | |
| 19:45 | | Holmer Green | | Shrivenham | | 3:2 | | |
| Holmer Green | | |
| 19:45 | | Winslow United | | Newport Pagnell Town | | 2:1 | | |
| Winslow | | |
| 19:45 | | Cribbs | | Newent Town | | 3:1 | | |
| Bristol | | |
| 19:45 | | Aylesbury Vale Dynamos | | Windsor | | 1:2 | | |
| Aylesbury | | |
| 19:45 | | Lydney Town | | Fairford Town | | 0:1 | | |
| Lydney | | |
| 19:45 | | Risborough Rangers | | Longlevens | | 4:1 | | |
| Princes Risborough | | |
| 19:45 | | AFC Dunstable | | Hallen | | 4:0 | | |
| Dunstable | | |
| 19:45 | | Royal Wootton Bassett Town | | Chipping Sodbury Town | | 2:2, pen. 4:1 | | |
| Wootton Bassett | | |
| 19:45 | | Burnham | | Cheltenham Saracens | | 0:0, pen. 5:3 | | |
| Burnham | | |
| 19:45 | | North Greenford United | | Brimscombe & Thrupp | | 1:2 | | |
| London | | |
| 19:45 | | CB Hounslow United | | Banstead Athletic | | 6:1 | | |
| London | | |
| 19:45 | | Billingshurst | | Westside | | 0:2 | | |
| Billingshurst | | |
| 19:45 | | Lordswood | | Hanworth Villa | | 2:3 | | |
| Chatham | | |
| 19:45 | | Molesey | | Southall | | 0:7 | | |
| Molesey | | |
| 19:45 | | AFC Croydon Athletic | | Sutton Common Rovers | | 0:3 | | |
| London | | |
| 19:45 | | Glebe | | Whyteleafe | | 1:3 | | |
| Bromley | | |
| 19:45 | | Broadbridge Heath | | Raynes Park Vale | | 3:1 | | |
| Broadbridge Heath | | |
| 19:45 | | Alfold | | Shoreham | | 9:1 | | |
| Alfold | | |
| 19:45 | | Saltdean United | | Eastbourne Town | | 1:3 | | |
| Saltdean | | |
| 19:45 | | Kennington | | Erith & Belvedere | | 0:1 | | |
| Ashford | | |
| 19:45 | | Langney Wanderers | | Tower Hamlets | | 3:1 | | |
| Eastbourne | | |
| 19:45 | | Abbey Rangers | | Welling Town | | 3:1 | | |
| Addlestone Addlestone Moor | | |
| 19:45 | | Oakwood | | Cobham | | 0:2 | | |
| Crawley | | |
| 19:45 | | Tunbridge Wells | | Erith Town | | 1:0 | | |
| Royal Tunbridge Wells | | |
| 19:45 | | Newhaven | | Lingfield | | 1:1, pen. 4:2 | | |
| Newhaven | | |
| 19:45 | | Broadfields United | | Loxwood | | 3:0 | | |
| London | | |
| 19:45 | | Redhill | | Egham Town | | -:+ | | |
| Redhill | | |
| 19:45 | | Fisher | | Horsham YMCA | | 2:0 | | |
| London | | |
| 19:45 | | Corinthian | | Sheerwater | | 2:1 | | |
| Hartley Gay Dawn Farm | | |
| 19:45 | | K Sports | | Steyning Town | | 0:2 | | |
| Aylesford | | |
| 19:45 | | Eastbourne United Association | | Horley Town | | 0:1 | | |
| Eastbourne | | |
| 19:45 | | Knaphill | | Deal Town | | 0:4 | | |
| Knaphill | | |
| 19:45 | | Bearsted | | Peacehaven & Telscombe | | 3:5 | | |
| Otham | | |
| 19:45 | | Spelthorne Sports | | Virginia Water | | 1:0 | | |
| Spelthorne | | |
| 19:45 | | Sheppey United | | Sutton Athletic | | 1:0 | | |
| Isle of Sheppey | | |
| 19:45 | | Corsham Town | | Farnham Town | | 2:2, pen. 4:3 | | |
| Corsham | | |
| 19:45 | | Hamworthy United | | Calne Town | | 3:0 | | |
| Poole | | |
| 19:45 | | Badshot Lea | | Amesbury Town | | 4:0 | | |
| Wrecclesham | | |
| 19:45 | | Hamble Club | | Reading City | | 2:1 | | |
| Hamble-le-Rice | | |
| 19:45 | | Frimley Green | | Fareham Town | | 1:0 | | |
| Frimley Green | | |
| 19:45 | | Bashley | | Whitchurch United | | 8:2 | | |
| Bashley | | |
| 19:45 | | Brockenhurst | | Lymington Town | | 0:1 | | |
| Brockenhurst | | |
| 19:45 | | Basingstoke Town | | AFC Bournemouth | | 1:1, pen. 5:4 | | |
| Reading | | |
| 19:45 | | Camberley Town | | Blackfield & Langley | | 0:0, pen. 5:4 | | |
| Camberley | | |
| 19:45 | | Shaftesbury | | Fawley | | 2:2, pen. 5:6 | | |
| Shaftesbury | | |
| 19:45 | | Tadley Calleva | | Alresford Town | | 1:0 | | |
| Tadley | | |
| 19:45 | | Bemerton Heath Harlequins | | Horndean | | 2:1 | | |
| Bemerton | | |
| 19:45 | | Christchurch | | Ascot United | | 1:1, pen. 7:6 | | |
| Christchurch | | |
| 19:45 | | AFC Portchester | | Sandhurst Town | | 4:0 | | |
| Portchester | | |
| 19:45 | | Keynsham Town | | Exmouth Town | | 1:3 | | |
| Keynsham | | |
| 19:45 | | Odd Down | | Helston Athletic | | 1:4 | | |
| Bath | | |
| 19:45 | | Wellington | | Bodmin Town | | 2:2, pen. 3:4 | | |
| Wellington | | |
| 19:45 | | Plymouth Parkway | | Saltash United | | 1:1, pen. 4:5 | | |
| Plymouth | | |
| 19:45 | | Millbrook | | Bridgwater Town | | 0:1 | | |
| Millbrook | | |
| 19:45 | | Newton Abbot Spurs | | AFC St Austell | | 3:0 (2:0) | | |
| Newton Abbot | | |
| 19:45 | | Oxhey Jets | | Bishop's Cleeve | | 1:0 | | |
| South Oxhey | | |
| 19:45 | | Heather St John's | | Walsall Wood | | 0:1 | | |
| Heather | | |
| 20:00 | | Thornaby | | Bridlington Town | | 2:0 | | |
| Thornaby-on-Tees | | |
| 20:00 | | Skelmersdale United | | Penistone Church | | 3:2 | | |
| Skelmersdale | | |
| 20:00 | | Kirkley & Pakefield | | Cogenhoe United | | 1:2 | | |
| Lowestoft | | |
| 20:00 | | Portland United | | Clevedon Town | | 0:1 | | |
| Isle of Portland | | |