 | 25.04.2001 17:30 | | KAMAZ (Naberezhnye Chelny) | – | Alnas (Almetyevsk) | | 2:0 (1:0) |
 | Naberezhnye Chelny |
| 25.04.2001 | | Amur-Energiya (Blagoveshchensk) | – | SKA-Energiya (Khabarovsk) | | 0:1 (0:0) |
| Blagoveshchensk |
| 25.04.2001 | | Dynamo (Barnaul) | – | Metallurg-Zabsib (Novokuznetsk) | | 1:0 (0:0) |
| Barnaul |
| 25.04.2001 | | Dynamo (Perm) | – | Dynamo-Mashinostroitel (Kirov) | | 1:3 (0:3) |
| |
| 25.04.2001 | | Energiya (Chaikovskiy) | – | Dynamo (Izhevsk) | | 0:1 (0:0) |
| |
| 25.04.2001 | | FK Tyumen (Tyumen) | – | Irtysh (Omsk) | | 1:0 (0:0) |
| Tyumen |
| 25.04.2001 | | Gazovik (Orenburg) | – | Nosta (Novotroitsk) | | 0:1 (0:1) |
| |
| 25.04.2001 | | Kuzbass-Dynamo (Kemerovo) | – | Shakhtyor (Prokopievsk) | | 3:0 (1:0) |
| Kemerovo |
| 25.04.2001 | | Okean (Nakhodka) | – | Luch (Vladivostok) | | 1:0 (0:0) |
| Nakhodka |
| 25.04.2001 | | Selenga (Ulan-Ude) | – | Zvezda (Irkutsk) | | 1:2 aet
(0:0, 1:1) |
| |
| 25.04.2001 | | Sibiryak (Bratsk) | – | Chkalovets-Olimpik (Novosibirsk) | | 0:1 (0:0) |
| |
| 25.04.2001 | | Sodovik (Sterlitamak) | – | Metallurg-Metiznik (Magnitogorsk) | | 2:1 (1:0) |
| Sterlitamak |
| 25.04.2001 | | UralAZ (Miass) | – | Spartak (Kurgan) | | 3:0 (1:0) |
| Miass UralAZ Stadium |
| 25.04.2001 | | Uralmash (Ekaterinburg) | – | FK Berezniki (Berezniki) | | 2:0 (1:0) |
| Yekaterinburg |
| 25.04.2001 | | Zenit (Chelyabinsk) | – | Uralets (Nizhniy Tagil) | | 3:1 (2:0) |
| Chelyabinsk |
| 25.04.2001 | | Chkalovets-1936 (Novosibirsk) | – | Dynamo (Omsk) | | 2:0 (0:0) |
| Novosibirsk |
| 20.04.2001 | | Lokomotiv (Saint Petersburg) | – | Volochanin-89 (Vyshniy-Volochek) | | 0:0 aet,
pen. 4:3 |
| Saint Petersburg |
| 19.04.2001 13:00 | | Avtomobilist (Noginsk) | – | Torpedo (Vladimir) | | 1:2 aet
(1:0, 1:1) |
| Noginsk |
| 19.04.2001 | | Arsenal-2 (Tula) | – | Lokomotiv (Kaluga) | | 0:2 (0:1) |
| |
| 19.04.2001 | | Avangard (Kursk) | – | Salyut-Energiya (Belgorod) | | 1:1 aet
(0:0),
pen. 3:4 |
| Kursk |
| 19.04.2001 | | Fabus (Bronnitsy) | – | FK Kolomna (Kolomna) | | 2:1 (1:0) |
| |
| 19.04.2001 | | FK Krasnoznamensk (Krasnoznamensk) | – | Titan (Reutov) | | 1:2 (0:0) |
| Selyatino |
| 19.04.2001 | | FK Oryol (Oryol) | – | Dynamo (Bryansk) | | 0:0 aet,
pen. 4:1 |
| Orel |
| 19.04.2001 | | FK Rybinsk (Rybinsk) | – | Severstal (Cherepovets) | | 0:2 (0:1) |
| Rybinsk |
| 19.04.2001 | | Kosmos (Elektrostal) | – | Spartak-Orekhovo (Orekhovo-Zuevo) | | 0:1 (0:0) |
| |
| 19.04.2001 | | Lokomotiv (Liski) | – | FK Yelyets (Yelyets) | | 1:0 (0:0) |
| |
| 19.04.2001 | | Lotto-MKM (Moscow) | – | Nika (Moscow) | | 1:0 (1:0) |
| |
| 19.04.2001 | | Mosenergo (Moscow) | – | Spartak (Shchyolkovo) | | 3:0 (2:0) |
| Moscow |
| 19.04.2001 | | Neftyanik (Yaroslavl) | – | Dynamo (Vologda) | | 1:2 (1:0) |
| Yaroslavl |
| 19.04.2001 | | Pskov-2000 (Pskov) | – | Krivichi (Velikiye Luki) | | 2:0 (1:0) |
| Pskov |
| 19.04.2001 | | Spartak (Lukhovitsy) | – | Agrokomplekt (Ryazan) | | 0:0 aet,
pen. 1:3 |
| Lukhovitsy |
| 19.04.2001 | | Sportakademklub (Moscow) | – | Oazis (Yartsevo) | | 5:1 (4:0) |
| Moscow |
| 19.04.2001 | | Vityaz (Podolsk) | – | Don (Novomoskovsk) | | 2:1 aet
(0:0, 1:1) |
| |
| 19.04.2001 | | Dynamo-SPb (Saint Petersburg) | – | Svetogorets (Svetogorsk) | | 2:1 aet
(0:0, 1:1) |
| Saint Petersburg |
| 19.04.2001 | | Spartak (Tambov) | – | Metallurg (Lipetsk) | | 3:1 (0:0) |
| Tambov |
| 19.04.2001 | | Spartak-Telekom (Shuya) | – | Spartak (Kostroma) | | 3:1 (1:1) |
| Shuya |
| 17.04.2001 | | Diana (Volzhsk) | – | Lada-Energiya (Dimitrovgrad) | | 1:1 aet
(1:0, 1:1),
pen. 4:5 |
| |
| 17.04.2001 | | Iskra (Engels) | – | Salyut (Saratov) | | 2:0 (2:0) |
| |
| 17.04.2001 | | Khoper (Balashov) | – | FK Balakovo (Balakovo) | | 1:0 (1:0) |
| |
| 17.04.2001 | | Olimpiya (Volgograd) | – | Torpedo (Volzhskiy) | | 2:1 (2:1) |
| |
| 17.04.2001 | | Sibur-Khimik (Dzerzhinsk) | – | Metallurg (Vyksa) | | 0:1 (0:0) |
| |
| 17.04.2001 | | Spartak (Yoshkar-Ola) | – | Electronika (Nizhniy Novgorod) | | 0:1 (0:0) |
| Yoshkar-Ola |
| 17.04.2001 | | Torpedo-Viktoriya (Nizhniy Novgorod) | – | Torpedo (Pavlovo) | | 1:1 aet
(0:1, 1:1),
pen. 4:2 |
| Nizhny Novgorod |
| 17.04.2001 | | Biokhimik-Mordovia (Saransk) | – | Svetotekhnika (Saransk) | | 0:2 (0:0) |
| Romodanovo Mayak Stadium |
| 24.03.2001 | | Nart (Nartkala) | – | Kavkazkabel (Prokhladnyi) | | 1:0 aet
(0:0) |
| |
| 24.03.2001 | | SKA (Rostov-on-Don) | – | Shakhtyor (Shakhty) | | 3:2 (2:1) |
| Rostov-on-Don |
| 24.03.2001 | | Spartak (Anapa) | – | Vityaz (Krymsk) | | 1:2 (0:2) |
| |
| 24.03.2001 | | Spartak-Kavkaztransgaz (Izobilny) | – | Dynamo (Stavropol) | | 2:1 (1:1) |
| Izobilny |
| 24.03.2001 | | Venets (Gulkevichi) | – | Lokomotiv-Taim (Mineralnye Vody) | | 3:0 (2:0) |
| Gulkevichi |
| 24.03.2001 | | Angusht (Nazran) | – | Terek (Groznyi) | | 0:1 (0:0) |
| Adler |
| 24.03.2001 | | Druzhba (Maykop) | – | Zhemchuzhina (Sochi) | | 0:1 (0:1) |
| Khosta |
| 24.03.2001 | | FK Krasnodar-2000 (Krasnodar) | – | Slavyansk (Slavyansk-na-Kubani) | | 2:0 (0:0) |
| Krasnodar |
| 24.03.2001 | | FK Mozdok (Mozdok) | – | Avtodor (Vladikavkaz) | | 3:1 (1:1) |
| Veseloye Iriston Stadium |
| 24.03.2001 | | Sudostroitel (Astrakhan) | – | Dynamo (Makhachkala) | | 1:2 (0:1) |
| Astrakhan |
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