FA Cup 2023/24, First Qualifying Round |
 | 05.09.2023 |  | Marine | – | Guiseley | | 3:0 (1:0) * |
| Crosby |
FA Cup 2022/23, Second Qualifying Round |
| 17.09.2022 | | Blyth Spartans | – | Guiseley | | 2:0 (1:0) |
| Blyth |
FA Cup 2021/22, First Round Proper |
| 06.11.2021 | | AFC Wimbledon | – | Guiseley | | 1:0 (1:0) |
| London |
FA Cup 2020/21, Fourth Qualifying Round |
| 24.10.2020 | | United of Manchester | – | Guiseley | | 2:1 |
| Bury |
FA Cup 2019/20, Third Qualifying Round |
| 05.10.2019 | | Peterborough Sports | – | Guiseley | | 1:0 (0:0) |
| Peterborough |
FA Cup 2018/19, Second Round Proper |
| 03.12.2018 | | Guiseley | – | Fleetwood Town | | 1:2 (1:2) |
| Guiseley |
FA Cup 2017/18, Second Round Proper |
| 03.12.2017 | | Mansfield Town | – | Guiseley | | 3:0 (1:0) |
| Mansfield |
FA Cup 2016/17, Fourth Qualifying Round |
| 18.10.2016 | | Guiseley | – | Lincoln City | | 1:2 * |
| Guiseley |
FA Cup 2015/16, Fourth Qualifying Round |
| 27.10.2015 | | Guiseley | – | Halifax Town | | 1:2 aet
(0:1, 1:1) * |
| Guiseley |
FA Cup 2014/15, Fourth Qualifying Round |
| 25.10.2014 | | Grimsby Town | – | Guiseley | | 3:0 (0:0) |
| Cleethorpes |
FA Cup 2013/14, Second Qualifying Round |
| 28.09.2013 | | Guiseley | – | Bradford Park Avenue | | 1:2 (0:2) |
| Guiseley |
FA Cup 2012/13, First Round Proper |
| 13.11.2012 | | Barrow | – | Guiseley | | 1:0 (0:0) * |
| Barrow-in-Furness |
FA Cup 2011/12, Third qualifying round |
| 15.10.2011 | | Ashington | – | Guiseley | | 1:0 |
| Ashington |
FA Cup 2010/11, First Round Proper |
| 06.11.2010 | | Guiseley | – | Crawley Town | | 0:5 (0:2) |
| Guiseley |
FA Cup 2009/10, Third Qualifying Round |
| 13.10.2009 | | Kendal Town | – | Guiseley | | 1:0 * |
| |
FA Cup 2008/09, Third Qualifying Round |
| 14.10.2008 | | Sheffield | – | Guiseley | | 2:1 * |
| |
FA Cup 2007/08, Third Qualifying Round |
| 13.10.2007 | | Guiseley | – | Vauxhall Motors | | 2:3 |
| |
FA Cup 2006/07, Third Qualifying Round |
| 14.10.2006 | | Guiseley | – | Newcastle Benfield Bay Plastics | | 0:1 |
| |
FA Cup 2005/06, First Qualifying Round |
| 10.09.2005 | | Guiseley | – | Salford City | | 0:1 |
| |
FA Cup 2004/05, First Qualifying Round |
| | | Chorley | – | Guiseley | | 2:1 * |
| |
FA Cup 2003/04, Second Qualifying Round |
| | | Runcorn Halton | – | Guiseley | | 3:0 |
| |
FA Cup 2002/03, First Round Proper |
| 16.11.2002 | | Luton Town | – | Guiseley | | 4:0 (3:0) |
| Luton |
FA Cup 2001/02, First Qualifying Round |
| | | Guiseley | – | Eccleshill United | | 1:2 |
| |
FA Cup 2000/01, First Qualifying Round |
| | | Guiseley | – | Farsley Celtic | | 0:1 |
| |
FA Cup 1999/00, First Round Proper |
| 30.10.1999 | | Forest Green Rovers | – | Guiseley | | 6:0 (4:0) |
| Nailsworth |
FA Cup 1998/99, Fourth Qualifying Round |
| | | Doncaster Rovers | – | Guiseley | | 3:1 |
| |
FA Cup 1997/98, Third Qualifying Round |
| | | Guiseley | – | Penrith | | 1:2 |
| |
FA Cup 1996/97, Second Qualifying Round |
| | | Morecambe | – | Guiseley | | 4:1 |
| |
FA Cup 1995/96, Fourth Qualifying Round |
| | | Blyth Spartans | – | Guiseley | | 2:0 |
| |
FA Cup 1994/95, First Round Proper |
| 13.11.1994 | | Guiseley | – | Carlisle United | | 1:4 (0:2) |
| Bradford |
FA Cup 1993/94, Second Qualifying Round |
| | | Winsford United | – | Guiseley | | 2:1 |
| |
FA Cup 1992/93, First Qualifying Round |
| | | Fleetwood Town | – | Guiseley | | 3:2 |
| |
FA Cup 1991/92, First Round Proper |
| 16.11.1991 | | Chester City | – | Guiseley | | 1:0 |
| |
FA Cup 1990/91, Preliminary Round |
| | | Hednesford Town | – | Guiseley | | 2:0 |
| |
FA Cup 1989/90, Preliminary Round |
| | | Langley Park | – | Guiseley | | 5:4 |
| |
FA Cup 1988/89, First Qualifying Round |
| | | Billingham Town | – | Guiseley | | 1:0 |
| |
FA Cup 1987/88, Preliminary Round |
| | | Ashington | – | Guiseley | | 3:1 |
| |
FA Cup 1986/87, Second Qualifying Round |
| | | Guiseley | – | Blyth Spartans | | 0:2 |
| |
FA Cup 1985/86, Preliminary Round |
| | | Rossendale United | – | Guiseley | | 1:0 |
| |
FA Cup 1984/85, First Qualifying Round |
| | | West Auckland Town | – | Guiseley | | 2:1 * |
| |
FA Cup 1983/84, First Qualifying Round |
| | | Morecambe | – | Guiseley | | 1:0 |
| |
FA Cup 1945/46, First Qualifying Round |
| | | Frickley Colliery | – | Guiseley | | 7:0 * |
| |
